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All answers having "Separate" as clue:

3 Character Answers:

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DEB



4 Character Answers:

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LONE

PART

REND

RIVE

SIFT

SORT



5 Character Answers:

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ALONE

APART

ASIDE

PLONK

RAVEL

SEVER

SPLIT

UNMIX



6 Character Answers:

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ASSORT

DETACH

DIVIDE

REMOVE

SLEAVE

SUNDER

UNGLUE

UNKNIT

UNLINK

UNPILE



7 Character Answers:

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ANALYSE

BREAKUP

DISBAND

DISJOIN

DISTILL

DIVERSE

DIVORCE

ISOLATE

SIFTOUT

SORTOUT

SPLITUP

UNHITCH



8 Character Answers:

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BOXEDOFF

CUTAPART

DISCRETE

DISSEVER

DISTINCT

ESTRANGE

UNCOUPLE



9 Character Answers:

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DISENGAGE

MOVEAPART

PULLAPART

SEGREGATE

UNRELATED



10 Character Answers:

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DISCONNECT

INDIVIDUAL



11 Character Answers:

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PARTCOMPANY

POMMELHORSE



15 Character Answers:

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PARTCOMPANYWITH